α-Farnesene production from lipid by engineered Yarrowia lipolytica

Abstract Producing high value-added products from waste lipid feedstock by microbial cell factory has great advantages to minimize the pollution as well improve economic value of wasted oils and fats. Yarrowia lipolytica is a non-conventional oleaginous yeast can grow on variety hydrophobic substrates. In this study, we explored its ability synthesize α-farnesene, an important sesquiterpene, using feedstock. Based α-farnesene production strain, constructed previously, identified that Erg12 was key limiting factor further increase production. The improved 35.8% through increasing copy number ERG12 FSERG20 oleic acid substrate. Expression heterologous VHb 12.7%. Combining metabolic engineering with optimization fermentation conditions, titer yield reached 10.2 g/L 0.1 g/g acid, respectively, in fed-batch cultivation. synthesis cooking oil other edible were also explored. Compared glucose carbon source, substrates obtained higher titer, but lower by-products accumulation, demonstrating advantage Y.